An enzyme, alphabetase, catalyzes this reaction: CD. Researchers find that the the Km for the substrate C is 7uM, and the Kcat is 800 s-1. The compound Y is found to be a very strong competitive inhibitor of the enzyme, with an ? of 5. In an experiment with the [E]T = 20uM, an amount of Y is added that reduces V0 to 8,000nM/s. What is [C] in this experiment? Recall that Kmapp = ?Km for a competitive inhibitor. How can you determine [C] with minimal calculation?
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